lentiviral grna library Search Results


mouse lentiviral crispr guide rna library v2  (Addgene inc)
93
Addgene inc mouse lentiviral crispr guide rna library v2
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Mouse Lentiviral Crispr Guide Rna Library V2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+grna+library/Genome-wide+Mouse+Lentiviral+CRISPR+gRNA+Library+v1+(Pooled+Library+%2350947)/bio_rxiv__2022__08__04__502755-176-7-14
Average 93 stars, based on 1 article reviews
mouse lentiviral crispr guide rna library v2 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
genome-scale lentiviral grna library  (CustomArray Inc)
90
CustomArray Inc genome-scale lentiviral grna library
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Genome Scale Lentiviral Grna Library, supplied by CustomArray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+grna+library/genome+scale+lentiviral+grna+library/pm26627737-243-0-40
Average 90 stars, based on 1 article reviews
genome-scale lentiviral grna library - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
gfp-labeled human whole-genome dual-grna lentiviral library  (VectorBuilder GmbH)
90
VectorBuilder GmbH gfp-labeled human whole-genome dual-grna lentiviral library
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Gfp Labeled Human Whole Genome Dual Grna Lentiviral Library, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+grna+library/gfp+labeled+human+whole+genome+dual+grna+lentiviral+library/pmc11868045__DataSheet1-5-6-12
Average 90 stars, based on 1 article reviews
gfp-labeled human whole-genome dual-grna lentiviral library - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pooled lentiviral guide rna (grna) libraries  (Paulmann Licht GmbH)
90
Paulmann Licht GmbH pooled lentiviral guide rna (grna) libraries
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Pooled Lentiviral Guide Rna (Grna) Libraries, supplied by Paulmann Licht GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+grna+library/pooled+lentiviral+guide+rna++grna++libraries/pm39506038-41-18-23
Average 90 stars, based on 1 article reviews
pooled lentiviral guide rna (grna) libraries - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
lentiviral guide rna (grna) library containing plasma membrane-localized proteins  (Corus Pharma Inc)
90
Corus Pharma Inc lentiviral guide rna (grna) library containing plasma membrane-localized proteins
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a <t>lentiviral</t> <t>CRISPR-library.</t> Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.
Lentiviral Guide Rna (Grna) Library Containing Plasma Membrane Localized Proteins, supplied by Corus Pharma Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+grna+library/lentiviral+guide+rna++grna++library+containing+plasma+membrane+localized+proteins/pm39116875-384-147-148
Average 90 stars, based on 1 article reviews
lentiviral guide rna (grna) library containing plasma membrane-localized proteins - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a lentiviral CRISPR-library. Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.

Journal: bioRxiv

Article Title: Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein1 (LRP1) as crucial cellular receptor

doi: 10.1101/2022.08.04.502755

Figure Lengend Snippet: Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a lentiviral CRISPR-library. Cells were treated three times with PMT(C1165S)DTa and surviving cells grown. Genomic DNA was extracted, inserted sgRNA amplified and sequenced.

Article Snippet: We utilized a well-functioning and validate genome-wide mouse lentiviral CRISPR guide RNA library v2 (Addgene).

Techniques: Stable Transfection, Expressing, Transduction, CRISPR, Amplification

The volcano plot shows gene expression changes between cells treated with lethal PMT-DTa chimera and those only transduced with the CRISPR library. All reads were mapped locally using BWA-MEM [ ,6], then quantified with featureCounts [4], and finally fold changes between the condition were calculated by DESeq2 [7] (see galaxy history). The volcano plot was drawn with the bioinfokit toolkit [8]. Significantly enriched genes, having a positive fold change above 0.584 and a p-value lower or equal 5%, are shown in blue. The significance thresholds are marked by gray-dotted lines. The ten most significant genes are highlighted with their name. Non-significant genes are colored in gray.

Journal: bioRxiv

Article Title: Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein1 (LRP1) as crucial cellular receptor

doi: 10.1101/2022.08.04.502755

Figure Lengend Snippet: The volcano plot shows gene expression changes between cells treated with lethal PMT-DTa chimera and those only transduced with the CRISPR library. All reads were mapped locally using BWA-MEM [ ,6], then quantified with featureCounts [4], and finally fold changes between the condition were calculated by DESeq2 [7] (see galaxy history). The volcano plot was drawn with the bioinfokit toolkit [8]. Significantly enriched genes, having a positive fold change above 0.584 and a p-value lower or equal 5%, are shown in blue. The significance thresholds are marked by gray-dotted lines. The ten most significant genes are highlighted with their name. Non-significant genes are colored in gray.

Article Snippet: We utilized a well-functioning and validate genome-wide mouse lentiviral CRISPR guide RNA library v2 (Addgene).

Techniques: Gene Expression, Transduction, CRISPR